Human DNA polymerase beta assay

DNA polymerase beta play key roles in the base excision repair (BER) process. It fills the small gaps (1 – 6 bases) of one DNA strand using the complementary strand as a template. Human DNA polymerase beta is an anti-cancer target. The human DNA polymerase beta assay is based on the principle that the repaired DNA forms more stable DNA duplex than the un-repaired one. The Assay Kit (catalog number DPB100K) is to measure the formation of the repaired DNA by its fluorescence signal in the presence of a fluorescence dye. Each kit includes the assay buffer, DNA template and fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.