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Drug Discovery, Drug Delivery, and Biochemical Assays
ProFoldin’s products and services are employed by universities, research institutes, biotech
companies, pharmaceutical R&D and research hospitals worldwide in life sciences and drug discovery.
E. coli Polynucleotide Phosphorylase (PNPase) Assay

Polynucleotide Polynucleotide Phosphorylase (PNPase) plays important roles in mRNA processing and degradation in various cells. RNA metabolism in bacteria involves posttranscriptional addition of poly(A) tails. PNPase has both exonuclease and polymerase activities. The E.coli PNPase assay is based on measurement of Poly(A) synthesized by E. coli PNPase using ADP.  The assay can be performed in a 384-well or 96-well plate format for tests of E. coli PNPase activities and high throughput screening of inhibitors. 

The E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit (Catalog No.  PNP100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x RNA, 33 µl of 100 x ADP (2.5 mM) and 330 µl of 10 x fluorescence dye. It is for 100 assays of E. coli PNPase in a 384-well plate format. The assay kit includes all reagents except the enzyme.     

The E. coli Polynucleotide Phosphorylase (PNPase) Assay Kit Plus (Catalog No.  PNP100KE) includes all the reagents for 100 assays of E. coli PNPase in a 384-well plate format. The kit includes 400 µl of 10 x Buffer, 33 µl of 100 x RNA, 33 µl of 100 x ADP (2.5 mM), 330 µl of 10 x fluorescence dye and 33 µl of 100 x E. coli PNPase (5 µM).