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DNA Polymerase Assay - DNA synthesis assay
DNA polymerase III synthesizes DNA using the RNA primer made by the DNA primase at the DNA replication fork of bacteria. DNA polymerase III alpha is the catalytic subunit of the polymerase. The DNA Polymerase III Alpha Assay Kit is based on measurement of the DNA molecules synthesized by the DNA polymerase. The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA polymerase inhibitors.
Each kit includes the assay reagents except the enzyme for 100 assays of DNA polymerase reactions in a 384-well assay format.
Each kit includes all the assay reagents for 100 assays of DNA polymerase reactions in a 384-well assay format. E. coli DNA polymerase III alpha is included.
Each kit includes all the assay reagents for 100 assays of DNA polymerase reactions in a 384-well assay format. H. influenzae DNA polymerase III alpha is included.
Each kit includes all the assay reagents for 100 assays of DNA polymerase reactions in a 384-well assay format. S. pneumoniae DNA polymerase III alpha is included.
References:
Hou Z et al, Selective in vivo and in vitro activities of 3,3'-4-nitrobenzylidene-bis-4-hydroxycoumarin against methicillin-resistant Staphylococcus aureus by inhibition of DNA polymerase III. Sci Rep. 2015 Sep 1;5:13637.
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