The bacterial DNA primase synthesizes RNA primers at the DNA replication fork where the DNA helicase (DnaB) unwinds the double strand DNA.   The Bacterial DNA Primase Assay Kits are based on measurement of the RNA primers synthesized by the DNA primase in the presence of the DNA temperate and DNA helicase.   The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA primase inhibitors. 

 E. coli DNA Primase Assay Kit                                Catalog No.  EGA100K      

Each kit includes the assay buffer and DNA template for 100 assays of DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format.  DNA primase assay kits for other bacterial strains are also available.