NAD⁺kinaseconverts NAD⁺into NADP⁺by phosphorylation in the presence of ATP.  NADP⁺plays key roles in energy transduction and various biochemical process including DNA repair, protein modification and cell signaling.  

 NAD⁺   +   ATP      =          NADP⁺   +    ADP

NAD⁺kinaseis an attractive antibacterial target.  The NAD⁺kinaseassay is based on measurement of ADP generated from the kinase reaction.  The assay is fluorescence-based and can be carried out using regular black 96-well or 384-well plates or micro-cuvettes.

E. coli NAD⁺Kinase Assay Kit Plus-100 (Catalog number: NAK100KE)

E. coli NAD⁺Kinase Assay Kit Plus-500 (Catalog number: NAK500KE)