The disease of tuberculosis (TB) is a serious threat to global public health.  It is estimated that infection of M. tuberculosis (Mtb) causes more than 1 million deaths annually. The protein degradation enzyme ClpP protease of Mtb is a proven drug target against Mtb.  ProFoldin’s high throughput assay for Mtb ClpP protease is based on cleavage of a labeled peptide substrate that generates fluorescence at 460 nm (excitation at 380 nm). The assay can be performed in a 384-well or 96-well plate format for tests of Mtb ClpP protease activities and throughput screening of inhibitors against M. tuberculosis. 

The M. Tuberculosis ClpP Protease Assay Kit (Catalog No.  TBP100K) includes 3100 µl of Assay Buffer and 31 µl of 100 x Substrate and 3500 µl of Stop solution. It is for 100 assays in 384-well plate format. All assay reagents except the enzyme are included.

The M. Tuberculosis ClpP Protease Assay Kit Plus (Catalog No.  TBP100KE) includes 3000 µl of Assay Buffer, 31 µl of 100 x Substrate, 3500 µl of Stop solution and 31 µl of 100 x Mtb ClpP1P2 protease. It is for 100 assays in 384-well plate format. All assay reagents are included.