NAD⁺-dependent DNA ligases are present in bacteria, some entomopox viruses and mimi virus. Since NAD⁺-dependent DNA ligases are essential for bacterial growth, they are valuable targets for identifying novel antibacterial agents. The NAD⁺-dependent DNA Ligase Assay Kit is to measure the DNA ligase product in which the diphosphodiester bond is formed at the single stand break of a duplex DNA substrate. The ligase reaction is monitored by the fluorescence intensity at 535 nm. The assay is in 96-well plate format and can be used for screening inhibitors of DNA ligases from gram-positive (such as S. pneumoniae) and gram-negative (such as E. coli) bacteria.

Each kit (Catalog number NLA100K) includes the assay buffer, DNA substrate, Reagent T and the fluorescence dye for 100 assays. Reagent T is used to stop the reaction and denature the DNA at the end of the ligase reaction.

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