Human mitochondrial protease ClpP has been identified as a drug target for human acute myeloid leukemia (AML). The high throughput assay for human ClpP protease is based on cleavage of a labeled peptide substrate that generates fluorescence at 460 nm (excitation at 380 nm). The assay can be performed in a 384-well or 96-well plate format for tests of Human ClpP protease activities and throughput screening of inhibitors for cancer therapy. 

The Human Mitochondrial ClpP Protease Assay Kit (Catalog No.  HMP100K) includes 3000 µl of Assay Buffer and 310 µl of 10 x Substrate and 3500 µl of Stop solution. It is for 100 assays in 384-well plate format. All assay reagents except the enzyme are included.

The Human Mitochondrial ClpP Protease Assay Kit Plus (Catalog No.  HMP100KE) includes 3000 µl of Assay Buffer, 31 µl of 100 x Substrate, 3500 µl of Stop solution and 31 µl of 100 x human ClpP protease. It is for 100 assays in 384-well plate format. All assay reagents are included.

References:

Jacques S. et al, Imipridone Anticancer Compounds Ectopically Activate the ClpP Protease and Represent a New Scaffold for Antibiotic Development. GENETICS vol. 214 no. 4 1103-1120 (2020).