Assays for DNA supercoiling, DNA decatenation, ATPase and DNA cleavage activities of E. coli and S. aureus topoisomerase II (gyrase) are available.  For assays of gyrases from other bacteria, please contact ProFoldin.

E. coli topoisomerase II (gyrase) assays

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus (enzyme included) (Catalog number: DSA020KE): For measurement of DNA supercoiling activity of topoisomerases in a 96-well or 384-well plate format; for 20 assays; E. coli gyrase is included.

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus-100 (enzyme included) (Catalog number: DSA100KE): For measurement of DNA supercoiling activity of topoisomerases in a 96-well or 384-well plate format; for 100 assays; E. coli gyrase is included.

DNA Topoisomerase II (Gyrase) Assay Reagent Set for HTS (enzyme not included) (Catalog number: DSA1000): Reagents for high throughput screen (HTS) of DNA gyrase inhibitors for the DNA supercoiling activity; for 1000 assays; DNA or enzyme is not included.

E. coli gyrase ATPase assay Kit Plus (enzyme included) (Catalog number: T2A-100KE): For measurement of E. coli gyrase ATPase activity in a 384-well plate format; for 100 assays; E. coligyrase is included.

96-well E. coli gyrase DNA decatenation assay Kit Plus (enzyme included) (Catalog number: T2DD-96KE): For measurement of E. coli gyrase DNA decatenation activity in a 96-well plate format; for 96 assays; E. coligyrase is included.

96 well E. coli Gyrase DNA Cleavage Assay Kit Plus (enzyme included) (Catalog number: T2C96KE): For measurement of DNA cleavage activity of gyrase drugs or inhibitors in a 96-well plate format; for 96 assays; E. coli gyrase is included.

S. aureus  topoisomerase II (gyrase) assays

S. aureus Gyrase DNA Supercoiling Assay Plus (enzyme included) (DSA020KSE): For measurement of DNA supercoiling activity of topoisomerases in a 96-well or 384-well plate format; for 20 assays; S. aureus gyrase is included.

S. aureus Gyrase DNA Supercoiling Assay Plus-100 (enzyme included) (DSA100KSE): For measurement of DNA supercoiling activity of topoisomerases in a 96-well or 384-well plate format; for 100 assays; S. aureus gyrase is included.

S. aureus gyrase ATPase assay Kit Plus (enzyme included) (Catalog number: T2A-100KS): For measurement of S. aureus gyrase ATPase activity in a 384-well plate format; for 100 assays; S. aureusgyrase is included.

96-well S. aureus gyrase DNA decatenation assay Kit Plus (enzyme included) (Catalog number: T2DD-96KS): For measurement of S. aureus gyrase DNA decatenation activity in a 96-well plate format; for 96 assays; S. aureus gyrase is included.

References:

1. Asha MK et al, In vitro anti-Helicobacter pylori activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action, Journal of Ethnopharmacology, Vol.145(2), pp.581-586 (2013).
2. Mora-Pale M. et al, Antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnol Bioeng.Vol 112, pp2417–2428 (2015).
3. "Tabrizi MA et al,Synthesis and Biological Evaluation of Pyrazolo[3,4-b]pyridin-4-ones as a New Class of Topoisomerase II Inhibitors. Medicinal Chemistry, 11, 342-353 (2015).
4. Khan UG, Developing a Lead Compound for the Inhibition of Bacterial DNA Gyrase. University of Toronto, Dissertations Publishing, (2016). 10192322.
5. Ushiyama F. ert al, Lead Identification of 8‑(Methylamino)-2-oxo-1,2-dihydroquinoline Derivatives as DNAGyrase Inhibitors: Hit-to-Lead Generation Involving Thermodynamic Evaluation. ACS Omega 5, 10145−10159 (2020).