DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA (DNA supercoiling reaction).   The Gel-Based E. coli Gyrase DNA Supercoiling Assay is based on the principle that the supercoiled DNA and relaxed DNA are separated by agarose gel electrophoresis.  Fluorescence-based DNA supercoiling assays in a 96-well pate format are also available for high throughput screening of gyrase inhibitors.  For more information of the high throughput gyrase DNA supercoiling assays, please visit the website at http://www.profoldin.com/topoisomerase_assays_1.html.

The Gel-Based E. coli Gyrase DNA Supercoiling Assay Kit Plus-100 (Catalog No. GDSA100KE) includes all the reagents for 100 samples in gel-based assays of E. coli gyrase DNA supercoiling activity.  It includes 400 µl of 10 x Buffer, 105 µl of 250 mg/ml relaxed DNA, 220 µl of 10 mM ATP, 550 µl of  5 x Gel loading buffer and 25 µl of 2000 nM E.coli gyrase (100 x).