DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA (DNA supercoiling reaction).   The Gel-Based Topoisomerase DNA Supercoiling Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA are separated by agarose gel electrophoresis.  Fluorescence-based DNA supercoiling assays in a 96-well pate format are also available for high throughput screening of gyrase inhibitors.  For more information of the high throughput gyrase DNA supercoiling assays, please visit the website at http://www.profoldin.com/topoisomerase_assays_1.html.

Gel-Based E. coli Topoisomerase II (Gyrase) DNA Supercoiling Assay Kit (Catalog No. GDSA100KE)

Gel-Based S. aureus Topoisomerase II (Gyrase) DNA Supercoiling Assay Kit (Catalog No. GDSA100KSE)

Gel-Based P. aeruginosa Topoisomerase II (Gyrase) DNA Supercoiling Assay Kit (Catalog No. GDSA100KPE)