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Drug Discovery, Drug Delivery, and Biochemical Assays
ProFoldin’s products and services are employed by universities, research institutes, biotech
companies, pharmaceutical R&D and research hospitals worldwide in life sciences and drug discovery.
DOPC Nanodiscs

Nanodiscs of phospholipids may be used for scientific research involving membrane proteins or cell membranes. The DOPC nanodisc are prepared by solubilizing a liposome composed of DOPC : cholesterol : DSPE-PEG2000 (7.5 : 1 : 2.5) using styrene - maleic acid (SMA) copolymers. The SMA to lipid mass ratio is 8:1.  After the liposome solubilization, the SMA 3:1 copolymer yields nanodiscs in an average diameter about 10 nm. The SMA 2:1 copolymer yields nanodiscs in an average diameter about 30 nm.  The total lipid concentration is 5 mg/ml and the SMA copolymer concentration is 40 mg/ml.  The nanodiscs are formed in a buffer composed of 10 mM Tris-HCl, pH 7.6.

10 nm DOPC Nanodisc (Catalog No. ND-OCGS-10) contains contains 0.31 ml of 32 mg/ml liposome composed of DOPC : cholesterol : DSPE-PEG2000 (mass ratio: 7.5 : 1 : 2.5) and 1.69 ml of 5 % maleic acid (SMA) 3:1 copolymer in 10 mM Tris-HCl, pH 7.6. To make the 10 nm nanodisc, mix the two reagents and incubate the mixture at room temperature overnight. 

30 nm DOPC Nanodisc (Catalog No. ND-OCGS-30) contains 0.31 ml of 32 mg/ml liposome composed of DOPC: cholesterol : DSPE-PEG2000 (mass ratio: 7.5 : 1 : 2.5) and 1.69 ml of 5 % maleic acid (SMA) 2:1 copolymer in 10 mM Tris-HCl, pH 7.6. To make the 30 nm nanodisc, mix the two reagents and incubate the mixture at room temperature overnight.