DNA topoisomerase I (topo I) converts supercoiled circular DNA into relaxed DNA (DNA relaxation reaction).  The Gel-Based E. coli Topoisomerase I DNA relaxation Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA are separated by agarose gel electrophoresis.  Fluorescence-based DNA relaxation assays in a 96-well pate format are also available for high throughput screening of E. coli topo I inhibitors.  For more information of the high throughput E. coli topo I assay, please visit the website at http://www.profoldin.com.

For high throughput E.coli DNA topo I DNA relaxation assay, please visit here.

The Gel-Based E. coli Topoisomerase I DNA relaxation Assay Kit (Catalog No. GDRA100KE) includes all the reagents for 100 samples in gel-based assays of E. coli topo I DNA relaxation activity.  It includes 300 µl of 10 x Buffer T1, 100 µl of 250 mg/ml supercoiled DNA, 550 µl of 5 x Gel loading buffer and 10 µl of 4 µM E. coli topoisomerase I enzyme.