Assays for DNA decatenation, DNA relaxation, ATPase and DNA cleavage activities of E. coli and S. aureus topoisomerase IV enzymes (parC-parE complex) are available.  For assays of topoisomerase IV enzymes from other bacteria, please contact ProFoldin.

E. coli topoisomerase IV (parC-parE complex) assays

E. coli DNA Topoisomerase IV Assay Kit Plus (enzyme included)  (Catalog number: DDC020KE): For measurement of DNA decatenation activity of topoisomerases in spin-column format; for 20 assays; E. coli Topo IV (parC-parE) is included.

E. coli DNA Topoisomerase IV Assay Kit Plus-100   (enzyme included) (Catalog number: DDC100KE): For measurement of DNA decatenation activity of topoisomerases in spin-column format; for 100 assays; E. coli Topo IV (parC-parE) is included.

96-well E. coli Topo IV DNA Decatenation Assay Kit Plus (enzyme included) (Catalog number: EDD96KE):   For measurement of DNA decatenation activity of topoisomerases in a 96-well plate format; for 96 assays; E. coli topo IV (parC-parE) is included.

E. coli Topo IV ATPase Assay Kit Plus  (enzyme included) (Catalog number: T4A-100KE):  For measurement of E. coliTopo IV ATPase activity in a 384-well plate format; for 100 assays; E. coli Topo IV (parC-parE) is included.o IV (parC-parE) drugs in a 96-well plate format; for 96 assays; E. coliTopo IV (parC-parE) is included.

E. coli Topo IV DNA Relaxation Assay Kit Plus (enzyme included) (Catalog number: T4RA-100KE):For measurement of DNA relaxation activity of topoisomerases in a 96-well or 384-well plate format; for 100 assays; E. coliTopo IV (parC-parE) is included.

96-well E. coli Topo IV DNA Cleavage Assay Kit Plus  (enzyme included) (Catalog number: T4C96KE): For measurement of DNA cleavage activity of E. coli  topo IV (parC-parE) drugs in a 96-well plate format; for 96 assays.

S. aureus topoisomerase IV (parC-parE complex) assays

96-well S. aureus Topo IV DNA Decatenation Assay Kit Plus (enzyme included) (Catalog number: SDD96KE): For measurement of DNA decatenation activity of topoisomerases in a 96-well plate format; for 96 assays; S. aureustopo IV (parC-parE) is included.

S. aureus Topoisomerase IV ATPase Assay Kit Plus  (enzyme included) (Catalog number: T4A-100KS):For measurement of S. aureusTopo IV ATPase activity in a 384-well plate format; for 100 assays; S. aureusTopo IV (parC-parE) is included.

96-well S. aureus Topo IV DNA Cleavage Assay Kit Plus (enzyme included) (Catalog number: T4C96KS):  For measurement of DNA cleavage activity of S. aureus topo IV (parC-parE) drugs in a 96-well plate format; for 96 assays; S. aureusTopo IV (parC-parE) is included.

S. aureus Topo IV DNA Relaxation Assay Kit Plus (enzyme included) (Catalog number: T4RA-100KS):For measurement of DNA relaxation activity of topoisomerases in a 96-well or 384-well plate format; for 100 assays; S. aureus Topo IV (parC-parE) is included.