DNA helicase (DnaB) hydrolyzes ATP as the source of molecular energy to carry out DNA unwinding required by the DNA replication process.  Inhibition of the ATPase activity of DNA helicase blocks its DNA unwinding function.  The DNA helicase ATPase assay can be used for high-throughput screen of DNA helicase inhibitors in drug discovery.  The DNA Helicase ATPase Assay Kit is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm.

E. coli DNA Helicase ATPase assay Kit Plus-100 (DNAB100KE)

E. coli DNA Helicase ATPase assay Kit Plus-500 (DNAB500KE)

P. aeruginosa DNA Helicase ATPase assay kit Plus-100 (DNAB100KP)

P. aeruginosa DNA Helicase ATPase assay kit Plus-500 (DNAB500KP)

H. influenzae DNA Helicase ATPase assay kit Plus-100 (DNAB100KH)

H. influenzae DNA Helicase ATPase assay kit Plus-500 (DNAB500KH)

S. pneumoniae DNA Helicase ATPase assay kit Plus-100 (DNAB100KN)

S. pneumoniae DNA Helicase ATPase assay kit Plus-500 (DNAB500KN)

S. aureus DNA Helicase ATPase assay Kit Plus-100 (DNAB100KS)

S. aureus DNA Helicase ATPase assay Kit Plus-500 (DNAB500KS)

References:                                               

Nakano T et al, Translocation and Stability of Replicative DNA Helicases upon Encountering DNA-Protein Cross-links, J. Biol. Chem. 288: 4649-4658 (2013).